Fresh seeds to sow

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How to GerminateCitrullus colocynthis

Citrullus colocynthis

Colocynth, Bitter apple, Bitter cucumber
Native · Mediterranean Basin, West Asia, North Africa, Arabian Desert
Difficulty
dificil
Temperature
25°C ideal
Germination
7–21days
after pre-treatment
Success rate
~60%
Light
escuro
Sow depth
1cm

Citrullus colocynthis is a desert perennial vine with deep seed dormancy. Seeds have strong physical dormancy due to mechanical barrier of testa. Germination is highly sensitive to light, temperature, and seed maturation conditions. Dormancy can be broken through scarification or gibberellic acid treatment

Before sowing

Scarification
mecanica Sandpaper scarification resulted in 61% germination. Physical scarification with H2SO4 achieved 98% germination after 4.5 hours

Germination highly dependent on seed maturation conditions. Seeds require chemical scarification (H2SO4) or mechanical scarification. Storage for 2 years improved germination from 58.8% to improved percentages

Sowing

Substrate
Sandy loam, well-draining soil, agar media
Depth
1cm
Temperature
25°C
Moisture
media
Container
Petri dishes or seed trays with sandy loam soil
Day/night swing
Alternating temperatures 25/35°C (light/dark) and 20/30°C show superior germination compared to constant temperatures. Optimal: 35°C light/15°C dark

What to expect

Step 1
Pre-treat the seed
Step 2
Sow at 1cm · 25°C
Day 7–21
Seedlings emerge (~60%)

Tips

Best results with chemical scarification (H2SO4 4.5h) or gibberellic acid (100 ppm) treatment. Germination is superior in continuous darkness at warm temperatures. Seeds collected from plants matured under warmer conditions show better germination. Avoid light exposure during germination. Seeds require warm temperatures (20-35°C) with higher temperatures producing faster germination

Sources: El-Keblawy et al. 2017, 2019; Saberi & Shahriari 2011; Sen & Bhandari 1974; Gharehmatrossian et al. 2014; Acta agriculturae Slovenica 2025

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